Catalog No: OKCD09353
Size:96WELLS
Price: $750.00
SKU
OKCD09353
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Datasheets/ManualsPrintable datasheet for PSRC1 ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proline/Serine Rich Coiled Coil protein 1 (PSRC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proline/Serine Rich Coiled Coil protein 1 (PSRC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proline/Serine Rich Coiled Coil protein 1 (PSRC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proline/Serine Rich Coiled Coil protein 1 (PSRC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proline/Serine Rich Coiled Coil protein 1 (PSRC1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proline/Serine Rich Coiled Coil protein 1 (PSRC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PSRC1 Microplate96 Wells (12 x 8 Well strips)
PSRC1 Lyophilized Standard2
100X PSRC1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.048ng/mL
Gene SymbolPSRC1
Gene Full Nameproline and serine rich coiled-coil 1
Alias SymbolsDDA3, FP3214
NCBI Gene Id84722
Protein NameProline/serine-rich coiled-coil protein 1
Description of TargetThis gene encodes a proline-rich protein that is a target for regulation by the tumor suppressor protein p53. The encoded protein plays an important role in mitosis by recruiting and regulating microtubule depolymerases that destabalize microtubules. Alternatively spliced transcript variants encoding different isoforms have been described.
Uniprot IDQ6PGN9
Protein Accession #NP_001005290.1
Nucleotide Accession #NM_001005290.3
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