Catalog No: OOPA00224 (Formerly GWB-BSP583)
Size:2UG
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for OOPA00224 |
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Product Format | Sterile Filtered clear solution. |
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Host | Escherichia Coli. |
:: | Product Introduction: PSME1 is an interferon gamma (IFNG) inducible proteasome activator essential for presentation of certain major histocompatibility (MHC) class I antigens. The PSME1 complex is an alternate proteasome activator which operates with no use of ubiquitin. The PSME1 complex is made out of two homologous subunits, alpha and beta, that functions in similar catalytic properties and is connected to a hexameric ring. Product Description: PSME1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 269 amino acids (1-249a.a.) and having a molecular mass of 30.8kDa. PSME1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. Please avoid freeze thaw cycles. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Peptide Sequence | MGSSHHHHHH SSGLVPRGSH MAMLRVQPEA QAKVDVFRED LCTKTENLLG SYFPKKISEL DAFLKEPALN EANLSNLKAP LDIPVPDPVK EKEKEERKKQ QEKEDKDEKK KGEDEDKGPP CGPVNCNEKI VVLLQRLKPE IKDVIEQLNL VTTWLQLQIP RIEDGNNFGV AVQEKVFELM TSLHTKLEGF HTQISKYFSE RGDAVTKAAK QPHVGDYRQL VHELDEAEYR DIRLMVMEIR NAYAVLYDII LKNFEKLKKP RGETKGMIY |
Formulation | The PSME1 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 0.1M NaCl and 10% glycerol. |
Purification | Greater than 90% as determined by SDS-PAGE. |
Gene Symbol | PSME1 |
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Alias Symbols | REG-alpha, PA28alpha, PA28a, Proteasome Activator subunit-1, Proteasome Activator 28 subunit alpha, Interferon gamma up-regulated I-5111 protein. |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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