PSME1 Antibody - middle region (ARP58519_P050)

Catalog No: ARP58519_P050

WB Suggested Anti-PSME1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human Lung

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Rabbit, Yeast
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PSME1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Guinea Pig: 93%; Human: 100%; Mouse: 86%; Pig: 100%; Rabbit: 93%; Rat: 93%; Yeast: 83%
• Peptide Sequence: Synthetic peptide located within the following region: KEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEI
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-PSME1 (ARP58519_P050) antibody is Catalog # AAP58519 (Previous Catalog # AAPP34822)
• Subunit: 1
• Reference: Lemaire,R., (2007) J. Proteome Res. 6 (11), 4127-4134

Target Info

• Gene Symbol: PSME1
• Gene Full Name: Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
• Alias Symbols: PA28A, IFI5111, REGalpha, PA28alpha, HEL-S-129m
• NCBI Gene Id: 5720
• Protein Name: Proteasome activator complex subunit 1
• Description of Target: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.
• Uniprot ID: Q06323
• Protein Accession #: NP_788955
• Nucleotide Accession #: NM_176783
• Protein Size (# AA): 250
• Molecular Weight: 28kDa
• Protein Interactions: HUWE1; PSME1; NAA10; PSMD14; SHFM1; UBC; PSME2; ADRM1; PSMA1; KIF5B; PARK2; BAG3; UBL7; PSRC1; PARVB; PHPT1; PRDX3; NUBP2; USP5; RBBP6; PSMD12; PSMD1; PSMC4; PSMC2; PSMB3; PSMB2; PSMB1; PSMA7; PSMA6; PSMA3; APP; OXCT1; CSE1L; GRB2; UBD; PSMD6; TUBG1; PSMB