PSMB8 ELISA Kit (Dog) (OKEH03975)

Catalog No: OKEH03975

Size:96T

Dog PSMB8 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PSMB8 ELISA Kit (Dog) (OKEH03975) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PSMB8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PSMB8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PSMB8 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Component:
  • PSMB8 Microplate: 96 Wells (12 x 8 Well strips)
  • PSMB8 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated PSMB8 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.064 ng/mL
• Specificity: Natural and recombinant Dog Proteasome subunit beta type-8
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PSMB8
• Gene Full Name: proteasome 20S subunit beta 8
• Alias Symbols: MHC class II DO beta;proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7);proteasome subunit beta 8;proteasome subunit beta type-8;proteasome subunit beta-5i.
• NCBI Gene Id: 474865
• Protein Name: Q5W416
• Description of Target: Proteasome subunit beta type-8
• Uniprot ID: https://www.uniprot.org/uniprot/Q5W416
• Protein Accession #: NP_001041550.1
• Nucleotide Accession #: NM_001048085.1
• Protein Size (# AA): 276
• Molecular Weight: 276 kDa