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PSMB8 Antibody (OAGA01026)

100 ul
$329.00
In Stock
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Predicted Species Reactivity:
Human
Product Format:
Liquid
Clonality:
Polyclonal
Isotype:
IgG
Host:
Rabbit
Application:
ICC, IF, IHC-FFPE, WB
Additional Information:
Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq]
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Positive Controls: A431, Molt-4, Raji
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Cellular Localization: Cytoplasm (By similarity), Nucleus
Reconstitution and Storage:
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Replacement Item:
This antibody may replace item sc-100284 from Santa Cruz Biotechnology.
Immunogen:
Recombinant fragment corresponding to a region within amino acids 52 and 276 of PSMB8 (Uniprot ID#P28062)
Purification:
Purified by antigen-affinity chromatography.
Concentration:
0.38 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-PSMB8 antibody - OAGA01026
Application Info:
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Storage Buffer:
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Gene Symbol:
PSMB8
Official Gene Full Name:
Proteasome subunit beta type-8
Alias Symbols:
D6S216 antibody, D6S216E antibody, LMP7 antibody, MGC1491 antibody, PSMB5i antibody, RING10 antibody, PSMB8 antibody, proteasome subunit Y2 antibody, macropain subunit C13 antibody, proteasome subunit beta type-8 antibody, really interesting new gene 10 p
NCBI Gene Id:
5696
Protein Name:
Proteasome subunit beta type-8
Swissprot Id:
P28062
Protein Accession #:
NP_004150.1
Molecular Weight:
30 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PSMB8.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PSMB8.

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