PSMB8 Antibody - N-terminal region (OAAB10897)

Catalog No: OAAB10897

Size:400ul

PSMB8 Antibody (N-term) (Cat. #OAAB10897) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PSMB8 antibody detected the PSMB8 protein (arrow).

PSMB8 is strongly supported by BioGPS gene expression data to be expressed in HL60

PSMB8 Antibody (N-term) (Cat. #OAAB10897) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC
• Additional Information: Precautions: PSMB8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes
  Cellular Location: Cytoplasm (By similarity). Nucleus (By similarity)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PSMB8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-36 amino acids from the N-terminal region of human PSMB8.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  PSMB8 is strongly supported by BioGPS gene expression data to be expressed in HL60

• Application Info: WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: PSMB8
• Alias Symbols: JMP, ALDD, LMP7, NKJO, D6S216, PRAAS1, PSMB5i, RING10, D6S216E
• NCBI Gene Id: 5696
• Protein Name: Proteasome subunit beta type-8
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P28062
• Protein Accession #: NP_004150.1
• Molecular Weight: 30354 Da