Catalog No: OKCD00992 (Formerly GWB-KBBTV3)
Size:96WELLS
Price: $675.00
SKU
OKCD00992
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Datasheets/ManualsPrintable datasheet for PSMA5 ELISA Kit (Human) (OKCD00992)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proteasome Subunit Alpha Type 5 (PSMa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proteasome Subunit Alpha Type 5 (PSMa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proteasome Subunit Alpha Type 5 (PSMa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proteasome Subunit Alpha Type 5 (PSMa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proteasome Subunit Alpha Type 5 (PSMa5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proteasome Subunit Alpha Type 5 (PSMa5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PSMA5 Microplate96 Wells (12 x 8 Well Strips)
PSMA5 Lyophilized Standard2 x 20 ng
100X Biotinylated PSMA5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.124 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Proteasome Subunit Alpha Type 5 (PSMa5). No significant cross-reactivity or interference between Proteasome Subunit Alpha Type 5 (PSMa5) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPSMA5
Gene Full Nameproteasome 20S subunit alpha 5
Alias Symbolsepididymis tissue sperm binding protein Li 11n;macropain subunit zeta;macropain zeta chain;multicatalytic endopeptidase complex zeta chain;proteasome (prosome, macropain) subunit, alpha type, 5;proteasome alpha 5 subunit;proteasome component 5;proteasome subunit alpha 5;proteasome subunit alpha type-5;proteasome subunit zeta;proteasome zeta chain;PSC5;ZETA.
NCBI Gene Id5686
Protein NameProteasome subunit alpha type-5
Description of TargetComponent of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Uniprot IDP28066
Protein Accession #NP_001186701
Nucleotide Accession #NM_001199772.1
Protein Size (# AA)241
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