PSMA Antibody (OASL01234)

Catalog No: OASL01234

Size:50ul

Immunohistochemical analysis of paraffin-embedded Prostate. 1, Antibody was diluted at 1:200 (4C, overnight) . 2, Citric acid, pH6.0 was used for antigen retrieval.

Immunohistochemical analysis of paraffin-embedded Prostatic carcinoma. 1, Antibody was diluted at 1:200 (4C, overnight) . 2, Citric acid, pH6.0 was used for antigen retrieval. 

Product Info

• Product Format: Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Monoclonal
• Isotype: IgG1, Kappa
• Host: Mouse
• Application: IHC-P
• Additional Information: Background: folate hydrolase 1(FOLH1) Homo sapiens this gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. 
  Function: alternative products: experimental confirmation may be lacking for some isoforms, catalytic activity: Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates, cofactor: binds 2 zinc ions per subunit. Required for NAALADase activity, domain: the NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal, enzyme regulation: the NAALADase activity is inhibited by beta-NAAG, quisqualic acid, 2-(phosphonomethyl) pentanedioic acid (PMPA) and EDTA. Activated by cobalt, Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC, Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates.
  Location: cytoplasmic, membranous
  Expression: Brain, Jejunum, Liver, Peripheral Nervous System, Prostate, Prostatic carcinoma.
• Reconstitution and Storage: Store at -20C for one year. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human Prostate-Specific Membrane Antigen (PSMA)
• Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Concentration: 1 mg/ml
• Specificity: This antibody detects endogenous levels of human Prostate-Specific Membrane Antigen (PSMA)
• Application Info: IHC-P 1:200

Target Info

• Gene Symbol: FOLH1
• Alias Symbols: PSM, FGCP, FOLH, GCP2, PSMA, mGCP, GCPII, NAALAD1, NAALAdase
• NCBI Gene Id: 2346
• Protein Name: Glutamate carboxypeptidase 2 (EC 3.4.17.21) (Cell growth-inhibiting gene 27 protein) (Folate hydrolase 1) (Folylpoly-gamma-glutamate carboxypeptidase) (FGCP) (Glutamate carboxypeptidase II) (GCPII) (M)
• Uniprot ID: Q04609