Catalog No: OKEH04198
Size:96T
Price: $525.00
SKU
OKEH04198
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Psip1 ELISA Kit (Mouse) (OKEH04198) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Psip1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Psip1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Psip1 captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.4% (n = 20)
Mean Inter-assay CV%: <= 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
Psip1 Microplate96 Wells (12 x 8 Well Strips)
Psip1 Standard2 x 2 ng
Sample Diluent1 x 20 mL
Psip1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.095 ng/mL
SpecificityNatural and recombinant Mouse PC4 and SFRS1-interacting protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPsip1
Gene Full NamePC4 and SFRS1 interacting protein 1
Alias SymbolsAA408851;AU015605;dense fine speckles 70;Dfs70;Ledgfa;Ledgfb;lens epithelium-derived growth factor;P;PC4 and SFRS1 interacting protein 2;PC4 and SFRS1-interacting protein;Psip2.
NCBI Gene Id101739
Protein NameQ99JF8
Description of TargetPC4 and SFRS1-interacting protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q99JF8
Protein Accession #NP_001277456.1
Nucleotide Accession #NM_001290527.1
Protein Size (# AA)528
Molecular Weight60 kDa
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