Catalog No: OKEH06656
Size:96T
Price: $625.00
SKU
OKEH06656
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Psip1 ELISA Kit (Bovine) (OKEH06656) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Psip1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Psip1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Psip1 captured in the well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Psip1 Microplate96 Wells (12 x 8 Well strips)
Psip1 Lyophilized Standard2 x 20 ng
100X Psip1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.083 ng/mL
SpecificityNatural and recombinant Bovine PC4 and SFRS1-interacting protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPSIP1
Gene Full NamePC4 and SFRS1 interacting protein 1
Alias Symbolslens epithelium-derived growth factor;PC4 and SFRS1-interacting protein.
NCBI Gene Id282011
Protein NameQ8MJG1
Description of TargetPC4 and SFRS1-interacting protein
Uniprot IDhttps://www.uniprot.org/uniprot/Q8MJG1
Protein Accession #NP_001193405
Nucleotide Accession #NM_001206476
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