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Catalog No: OKEH02291
Size:96T
Price: $525.00
SKU
OKEH02291
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PSG11 ELISA Kit (Human) (OKEH02291) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PSG11 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PSG11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PSG11 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 10.7% (n = 20)
ELISA Kit Component
ComponentAmount
PSG11 Microplate96 Wells (12 x 8 Well strips)
PSG11 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PSG11 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.24 ng/mL
SpecificityNatural and recombinant Human Pregnancy-specific beta-1-glycoprotein 11
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPSG11
Gene Full Namepregnancy specific beta-1-glycoprotein 11
Alias Symbolspregnancy-specific beta-1-glycoprotein 11;pregnancy-specific beta-1-glycoprotein 13;PS-beta-G-11;PS-beta-G-13;PSBG-11;PSBG-13;PSG13;PSG14.
NCBI Gene Id5680
Protein NameQ9UQ72
Description of TargetPregnancy-specific beta-1-glycoprotein 11
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UQ72
Protein Accession #NP_001106881.1
Nucleotide Accession #NM_001113410.1
Protein Size (# AA)335
Molecular Weight37 kDa
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