PSEN1 ELISA Kit (Human) (OKEH08321)

Catalog No: OKEH08321

Size:96T

Human PSEN1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology PSEN1 ELISA Kit (Human) (OKEH08321) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PSEN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PSEN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PSEN1 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.7% (n = 20)
  Mean Inter-assay CV%: <=10.0% (n = 20)
• ELISA Kit Component:
  • PSEN1 Microplate: 96 Wells (12 x 8 Well strips)
  • PSEN1 Lyophilized Standard: 2
  • 100X Biotinylated PSEN1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein) (PubMed:15274632, PubMed:10545183, PubMed:10593990, PubMed:10206644, PubMed:10899933, PubMed:10811883, PubMed:12679784, PubMed:12740439, PubMed:25043039, PubMed:26280335). Requires the presence of the other members of the gamma-secretase complex for protease activity (PubMed:15274632, PubMed:25043039, PubMed:26280335). Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (PubMed:9738936, PubMed:10593990, PubMed:10899933, PubMed:10811883). Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin) (PubMed:11953314). Under conditions of apoptosis or calcium influx, cleaves CDH1 (PubMed:11953314). This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (PubMed:9738936, PubMed:11953314). Required for normal embryonic brain and skeleton development, and for normal angiogenesis.
  Subcellular Location: Endoplasmic reticulum membrane Multi-pass membrane protein Golgi apparatus membrane Multi-pass membrane protein Cytoplasmic granule Cell membrane Translocates with bound NOTCH1 from the endoplasmic reticulum and/or Golgi to the cell surface (PubMed:10593990). Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane (PubMed:9738936). Also present in azurophil granules of neutrophils (PubMed:11987239). Colocalizes with UBQLN1 in the cell membrane and in cytoplasmic juxtanuclear structures called aggresomes (PubMed:21143716).
  Unigene: Hs.3260
  SMR: P49768
  String: 9606.ENSP00000326366
  KEGG: hsa:5663
  MIM: 104311
  Pfam: PF01080
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 21pg/mL
• Specificity: Natural and recombinant Human Presenilin-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PSEN1
• Gene Full Name: presenilin 1
• Alias Symbols: ACNINV3;AD3;FAD;presenilin-1;Protein S182;PS1;PS-1;S182.
• NCBI Gene Id: 5663
• Protein Name: P49768
• Description of Target: Presenilin-1
• Uniprot ID: https://www.uniprot.org/uniprot/P49768
• Protein Accession #: NP_000012.1
• Nucleotide Accession #: NM_000021.3