PSCA ELISA Kit (Human) (OKCD08237)

Catalog No: OKCD08237

Size:96WELLS

PSCA ELISA Kit (Human) (OKCD08237) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostate Stem Cell Antigen (PSCA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostate Stem Cell Antigen (PSCA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostate Stem Cell Antigen (PSCA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Prostate Stem Cell Antigen (PSCA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostate Stem Cell Antigen (PSCA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostate Stem Cell Antigen (PSCA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • PSCA Microplate: 96 Wells (12 x 8 Well strips)
  • PSCA Lyophilized Standard: 2
  • 100X PSCA HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.063ng/mL

Target Info

• Gene Symbol: PSCA
• Gene Full Name: prostate stem cell antigen
• Alias Symbols: PRO232;prostate stem cell antigen.
• NCBI Gene Id: 8000
• Protein Name: Prostate stem cell antigen
• Description of Target: May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro.
• Uniprot ID: O43653