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Catalog No: OKDD00486 (Formerly GWB-KBBJP9)
Size:96 Wells
Price: $800.00
SKU
OKDD00486
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Datasheets/ManualsPrintable datasheet for PSAT1 ELISA Kit (Human) (OKDD00486)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology PSAT1 ELISA Kit (Human) (OKDD00486) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for PSAT1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PSAT1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PSAT1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PSAT1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PSAT1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-PSAT1 Microplate96 Wells (12 x 8 Well Strips)
PSAT1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X PSAT1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Assay Diluent1 x 45 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates
Sensitivity< 0.064 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of PSAT1. No significant cross-reactivity or interference between PSAT1 and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolPSAT1
Gene Full Namephosphoserine aminotransferase 1
Alias Symbolsendometrial progesterone-induced protein;EPIP;NLS2;phosphohydroxythreonine aminotransferase;phosphoserine aminotransferase;phosphoserine transaminase;PSA;PSAT;PSATD.
NCBI Gene Id29968
Protein NamePhosphoserine aminotransferase
Description of TargetCatalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.
Uniprot IDQ9Y617
Protein Accession #NP_066977.1
Nucleotide Accession #NM_021154.4
Protein Size (# AA)370
Molecular Weight40 kDa
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