- Predicted Species Reactivity:
- Product Format:
- 1 x 96-Well Plate or 12 x 8-Well Strips
- Kit Detection Method:
- Colorimetric, OD450 nm
- Kit Principle:
- The Aviva Human PSA ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hPSA concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human PSA cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
- Kit Range:
- 938-6,0000 pg/mL
- Kit Component:
|Capture Antibody Coated Microplate||96 Wells (12 x 8)|
|Ready-to-Use Streptavidin-HRP||12 mL|
|Ready-to-Use Substrate||12 mL|
|Stop Solution||12 mL|
|Wash Buffer (15x)||50 mL|
|Protein Standard Diluent||12 mL|
|Sample Diluent||12 mL|
|Detection Antibody Diluent||12 mL|
|Biotin-Labeled Detection Antibody||Lyophilized|
|Protein Standard||Lyophilized (110 ng)|
- Reconstitution and Storage:
- Store as indicated in product manual.
- Sample Type:
- Cell lysates, sera and plasma
- 938 pg/mL
- Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
- The Human PSA ELISA is capable of recognizing both recombinant and naturally produced Human PSA proteins. The antigens listed below were tested at 600 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: Kallikrein 2, A2-macroglobulin
- Assay Info:
- Quantitative Colorimentric Sandwich ELISA
- Official Gene Full Name:
- kallikrein related peptidase 3
- Alias Symbols:
- APS, Gamma-seminoprotein, hK3, Kallikrein-3, KLK2A1, P-30 antigen, Prostate-specific antigen, PSA, Semenogelase, Seminin
- NCBI Gene Id:
- Protein Name:
- Prostate-specific antigen
- Description of Target:
- Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms.
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express KLK3.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express KLK3.