Catalog No: OKEH01879
Size:96T
Price: $600.00
SKU
OKEH01879
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRTN3 ELISA Kit (Human) (OKEH01879) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRTN3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRTN3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRTN3 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 6.2% (n = 20)
ELISA Kit Component
ComponentAmount
PRTN3 Microplate96 Wells (12 x 8 Well strips)
PRTN3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PRTN3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant Human Myeloblastin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPRTN3
Gene Full Nameproteinase 3
Alias SymbolsACPA;AGP7;azurophil granule protein 7;CANCA;C-ANCA;C-ANCA antigen;leukocyte proteinase 3;MBN;MBT;myeloblastin;neutrophil proteinase 4;NP4;NP-4;P29;PR3;PR-3;serine proteinase, neutrophil;wegener autoantigen;Wegener granulomatosis autoantigen.
NCBI Gene Id5657
Protein NameP24158
Description of TargetMyeloblastin
Uniprot IDhttps://www.uniprot.org/uniprot/P24158
Protein Accession #NP_002768.3
Nucleotide Accession #NM_002777.3
Protein Size (# AA)256
Molecular Weight27 kDa
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