Catalog No: OKEH00323
Size:96T
Price: $525.00
SKU
OKEH00323
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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PRSS2 ELISA Kit (Rat) : 96 Wells (OKEH00323)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRSS2 ELISA Kit (Rat) (OKEH00323) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRSS2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for PRSS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRSS2 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)
ELISA Kit Component
ComponentAmount
PRSS2 Microplate96 Wells (12 x 8 Well strips)
PRSS2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PRSS2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.87 ng/mL
SpecificityNatural and recombinant Rat Anionic trypsin-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPrss2
Gene Full Nameprotease, serine, 2
Alias Symbolsanionic trypsin II;anionic trypsin-2;pancreatic trypsin 2;pancreatic trypsin II;pretrypsinogen II;Ptryss2;serine protease 2.
NCBI Gene Id25052
Protein NameP00763
Description of TargetAnionic trypsin-2
Uniprot IDhttps://www.uniprot.org/uniprot/P00763
Protein Accession #NP_036861.1
Nucleotide Accession #NM_012729.1
Protein Size (# AA)246
Molecular Weight26 kDa
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