Catalog No: OKEH03417
Size:96T
Price: $525.00
SKU
OKEH03417
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRSS1 ELISA Kit (Rat) (OKEH03417) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prss1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Prss1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prss1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit Component
ComponentAmount
Anti-Prss1 Microplate96 Wells (12 x 8 Well strips)
Prss1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Prss1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity40.6 pg/mL
SpecificityNatural and recombinant Rat Anionic trypsin-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPrss1
Gene Full Nameserine protease 1
Alias Symbolsanionic trypsin I;anionic trypsin-1;pancreatic trypsin 1;pretrypsinogen I;protease, serine 1;protease, serine, 1 (trypsin 1);protease, serine, 2;PTRYI;RATPTRYI;Serine protease 1;Try1.
NCBI Gene Id24691
Protein NameP00762
Description of TargetAnionic trypsin-1
Uniprot IDhttps://www.uniprot.org/uniprot/P00762
Protein Accession #NP_036767.1
Nucleotide Accession #NM_012635.1
Protein Size (# AA)246
Molecular Weight26 kDa
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