PRPSAP1 ELISA Kit (Mouse) (OKEH05564)

Catalog No: OKEH05564

Size:96 Wells

Mouse PRPSAP1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Prpsap1 ELISA Kit (Mouse) (OKEH05564) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prpsap1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prpsap1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prpsap1 captured in the well.
• ELISA Kit Range: 78 - 5000pg/ml
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 112.7%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <8.4%, n = 20
  Mean Inter-Assay Precision - <10.5%, n = 20
• ELISA Kit Component:
  • PRPSAP1 Microplate: 96 Wells (12 x 8 Well strips)
  • PRPSAP1 Lyophilized Standard: 2
  • 100X Biotinylated PRPSAP1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 39.9 pg/mL
• Specificity: Natural and recombinant Mouse Phosphoribosyl pyrophosphate synthase-associated protein 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PRPSAP1
• Gene Full Name: Phosphoribosyl pyrophosphate synthetase-associated protein 1
• Alias Symbols: PA, PAP39, 5730409F23Rik
• NCBI Gene Id: 67763
• Protein Name: Phosphoribosyl pyrophosphate synthase-associated protein 1
• Description of Target: Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis.
• Uniprot ID: Q9D0M1
• Protein Accession #: NP_080640.1
• Nucleotide Accession #: NM_026364.1
• Protein Size (# AA): 356