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Catalog No: OKEH02293
Size:96T
Price: $525.00
SKU
OKEH02293
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRPSAP1 ELISA Kit (Human) (OKEH02293) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRPSAP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRPSAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRPSAP1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 9.7% (n = 20)
ELISA Kit Component
ComponentAmount
PRPSAP1 Microplate96 Wells (12 x 8 Well strips)
PRPSAP1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PRPSAP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Phosphoribosyl pyrophosphate synthase-associated protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPRPSAP1
Gene Full Namephosphoribosyl pyrophosphate synthetase associated protein 1
Alias Symbols39 kDa phosphoribosypyrophosphate synthase-associated protein;PAP39;phosphoribosyl pyrophosphate synthase-associated protein 1;PRPP synthase-associated protein 1.
NCBI Gene Id5635
Protein NameQ14558
Description of TargetPhosphoribosyl pyrophosphate synthase-associated protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q14558
Protein Accession #NP_002757.2
Nucleotide Accession #NM_002766.2
Protein Size (# AA)356
Molecular Weight39 kDa
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