Catalog No: OKEH03505
Price: $675.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03505
Product Info
Predicted Species ReactivityHomo sapiens, Human
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRPH ELISA Kit (Human) (OKEH03505) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PRPH antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PRPH and incubated. The PRPH found in the sample or standards competes with the biotinylated PRPH for limited binding sites on the immobilized anti-human PRPH antibody. Excess unbound biotinylated PRPH and sample or standard PRPH is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PRPH captured in the well and inversely proportional to the amount of PRPH which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/ml
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 5.6% (n = 20)
ELISA Kit Component
Anti-PRPH Microplate96 Wells (12 x 8 Well strips)
PRPH Lyophilized Standard2
Sample Diluent1 x 20 mL
100X PRPH-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Human Peripherin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolPRPH
Gene Full Nameperipherin
Alias SymbolsNEF4;Neurofilament 4;neurofilament 4 (57kD);peripherin;PRPH1.
NCBI Gene Id5630
Protein NameP41219
Description of TargetPeripherin
Uniprot ID
Protein Accession #NP_006253.2
Nucleotide Accession #NM_006262.3
Protein Size (# AA)470
Molecular Weight54 kDa
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