Protocadherin Gamma (pan) Antibody : Biotin (OASE00302-Biotin)

Catalog No: OASE00302-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: S159-5
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: WB, IHC
• Additional Information: Background Info: Detects a 100kDa protein. Cross-reacts with all Gamma-
  protocadherin-A, -B and -C proteins.
  
  Scientific Background: The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes.
  The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
  
  Certificate of Analysis: 1ug/mL was sufficient for detection of Protocadherin gamma (pan) in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A subfamily amino acids ~807-930, B subfamily amino acids ~789-912 and C subfamily amino acids ~818-941). 99% identity with human (123/124 amino acids).
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 WB
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide

Target Info

• Gene Symbol: Pcdhga1
• Gene Full Name: protocadherin gamma subfamily A, 1
• NCBI Gene Id: 93709
• Uniprot ID: Q91XZ0
• Protein Accession #: NP_291062.1