Protein Kinase A regulatory subunit I alpha Antibody - N-terminal region (OAAB17462)

Catalog No: OAAB17462

Size:400ul

Western blot analysis of anti-PKR1 Antibody (N-term) (Cat.# OAAB17462) in CEM cell line lysates (35ug/lane). PKR1 (arrow) was detected using the purified Pab.

There is BioGPS gene expression data showing that PRKAR1A is expressed in HEK293

Western blot analysis of PKR1 (arrow) using rabbit polyclonal hPKR1-M1 (Cat. # OAAB17462). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKR1 gene (Lane 2).(2ug/ml)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: Protein Kinase A regulatory subunit I alpha Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells
  Cellular Location: Cell membrane.
  Tissue Location: Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Protein Kinase A regulatory subunit I alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Protein Kinase A regulatory subunit I alpha.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that PRKAR1A is expressed in HEK293

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: PRKAR1A
• Alias Symbols: CAR, CNC, CNC1, PKR1, TSE1, ADOHR, PPNAD1, PRKAR1, ACRDYS1
• NCBI Gene Id: 5573
• Protein Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P10644
• Protein Accession #: NP_001263218.1
• Molecular Weight: 42982 Da