Protein C Polyclonal Antibody (OABB00634)

Catalog No: OABB00634 (Formerly GWB-BBP557)

Size:100UG

Lane1: Jurkat Cell Lysate
Lane2: CEM Cell Lysate
Lane3: SMMC Cell Lysate
Lane4: Hela Cell Lysate

IHC(P):Human Mammary Cancer Tissue

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Flow cytometry|Immunocytochemistry|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Protein C(PROC), also called PC, is a zymogenic(inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped on 2q14.3. The PROC gene contains 8 exons and spans about 11 kb by Foster et al. The conversion of protein C to a protease with anticoagulant function by thrombin requires as a cofactor thrombomodulin, an endothelial cell membrane protein. Riewald et al. demonstrated that activated protein C uses the endothelial cell protein C receptor as a coreceptor for cleavage of protease-activated receptor-1 on endothelial cells. Faust et al. demonstrated that the endothelial pathways required for protein C activation are impaired in severe meningococcal sepsis. They stated that improvement in the outcome of children with meningococcal sepsis who were treated with unactivated protein C concentrates had been described in case reports and in 1 uncontrolled series.
  Background: Protein C(PROC), also called PC, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped on 2q14.3. The PROC gene contains 8 exons and spans about 11 kb by Foster et al. The conversion of protein C to a protease with anticoagulant function by thrombin requires as a cofactor thrombomodulin, an endothelial cell membrane protein. Riewald et al. demonstrated that activated protein C uses the endothelial cell protein C receptor as a coreceptor for cleavage of protease-activated receptor-1 on endothelial cells. Faust et al. demonstrated that the endothelial pathways required for protein C activation are impaired in severe meningococcal sepsis. They stated that improvement in the outcome of children with meningococcal sepsis who were treated with unactivated protein C concentrates had been described in case reports and in 1 uncontrolled series.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
  Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human
  Immunocytochemistry: 0.5-1 ug/ml: Human
  Flow Cytometry: 1-3 ug/1x10^6 cells: Human
• Reference: 1. Allaart, C. F., Poort, S. R., Rosendaal, F. R., Reitsma, P. H., Bertina, R. M., Briet, E. Increased risk
  of venous thrombosis in carriers of hereditary protein C deficiency defect. Lancet 341: 134-138,
  1993.
  2. Beckmann, R. J., Schmidt, R. J., Santerre, R. F., Plutzky, J., Crabtree, G. R., Long, G. L. The
  structure and evolution of a 461 amino acid human protein C precursor and its messenger RNA,
  based upon the DNA sequence of cloned human liver cDNAs. Nucleic Acids Res. 13: 5233-5247,
  1985.
  3. Berg, L.-P., Scopes, D. A., Alhaq, A., Kakkar, V. V., Cooper, D. N. Disruption of a binding site for
  hepatocyte nuclear factor 1 in the protein C gene promoter is associated with hereditary
  thrombophilia. Hum. Molec. Genet. 3: 2147-2152, 1994.
  FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Vitamin K-dependent protein C(PROC) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human.

Target Info

• Gene Symbol: PROC
• Gene Full Name: protein C, inactivator of coagulation factors Va and VIIIa
• Alias Symbols: activated protein C;anticoagulant protein C;APC;autoprothrombin IIA;blood coagulation factor XIV;PC;prepro-protein C;PROC1;Protein C-Nagoya;THPH3;THPH4;type I protein C;vitamin K-dependent protein C.
• NCBI Gene Id: 5624
• Protein Name: Vitamin K-dependent protein C
• Description of Target: Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
• Uniprot ID: P04070
• Molecular Weight: 52071 MW