PROTEIN A PEROXIDASE Conjugated (OORA00142)

Catalog No: OORA00142

Size:2MG

Product Info

• Predicted Species Reactivity: Staphylococcus aureus
• Product Format: Lyophilized
• Conjugation: HRP
• Application: Dot blot, Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot
• Reconstitution and Storage: Restore with 1.0 mL of deionized water (or equivalent). Store vial at 4°C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Concentration: 2 mg/ml
• Specificity: Protein A Peroxidase Conjugated was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.
• Application Info: Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate.
  All assays should be optimized by the user. Recommcended dilutions may be listed below.
  The suggested dilutions are: ELISA: 1:20,000 - 1:200,000 WB: 1:10,000 - 1:40,000 IHC: 1:1,000 - 1:5,000
  
• Purification: Protein A Peroxidase Conjugated was prepared from chromatographically pure Protein A.

Target Info

• Alias Symbols: Staphylococcal protein A.
• Protein Name: P38507
• Description of Target: Immunoglobulin G-binding protein A
• Uniprot ID: https://www.uniprot.org/uniprot/P38507
• Molecular Weight: 40000