Prostaglandin F2alpha ELISA Kit (OKEH02618)

Catalog No: OKEH02618

Size:96T

Prostaglandin F2alpha ELISA Kit (OKEH02618) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Prostaglandin F2alpha ELISA Kit (OKEH02618) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Prostaglandin F2alpha antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Prostaglandin F2alpha and incubated. The Prostaglandin F2alpha found in the sample or standards competes with the biotinylated Prostaglandin F2alpha for limited binding sites on the immobilized anti-Prostaglandin F2alpha antibody. Excess unbound biotinylated Prostaglandin F2alpha and sample or standard Prostaglandin F2alpha is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Prostaglandin F2alpha captured in the well and inversely proportional to the amount of Prostaglandin F2alpha which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 101%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.1% (n = 20)
  Mean Inter-assay CV%: < 8.5% (n = 20)
• ELISA Kit Component:
  • Anti-Prostaglandin F2alpha Microplate: 96 Wells (12 x 8 Well strips)
  • Prostaglandin F2alpha Lyophilized Standard: 2 x 25 ng
  • Sample Diluent: 1 x 20 mL
  • 100X Prostaglandin F2alpha-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: PGF2A
  Target Name: Prostaglandin F2alpha
  Pubchem: 5280363
  Chemical formula: C20H34O5
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 0.065 ng/mL
• Specificity: Natural and recombinant General Prostaglandin F2alpha
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: PGF2A, Prostaglandin F2alpha, PGF2A
• Protein Name: Prostaglandin F2alpha
• Description of Target: Prostaglandin F2alpha is produced by the uterus when stimulated by oxytocin, in the event that there has been no implantation during the follicular phase. It acts on the corpus luteum to cause luteolysis, forming a corpus albicans and stopping the production of progesterone. Action of PGF2alpha is dependent on the number of receptors on the corpus luteum membrane.