Prostaglandin D2 ELISA Kit (OKEH02602)

Catalog No: OKEH02602

Size:96T

Prostaglandin D2 ELISA Kit (OKEH02602) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Prostaglandin D2 ELISA Kit (OKEH02602) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Prostaglandin D2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Prostaglandin D2 and incubated. The Prostaglandin D2 found in the sample or standards competes with the biotinylated Prostaglandin D2 for limited binding sites on the immobilized anti-Prostaglandin D2 antibody. Excess unbound biotinylated Prostaglandin D2 and sample or standard Prostaglandin D2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Prostaglandin D2 captured in the well and inversely proportional to the amount of Prostaglandin D2 which was contained in the sample or standard.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.1% (n = 20)
  Mean Inter-assay CV%: < 8.7% (n = 20)
• ELISA Kit Component:
  • Anti-Prostaglandin D2 Microplate: 96 Wells (12 x 8 Well strips)
  • Prostaglandin D2 Lyophilized Standard: 2 x 2.5 ng
  • Sample Diluent: 1 x 20 mL
  • 100X Prostaglandin D2-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: PGD2
  Target Name: Prostaglandin D2
  Pubchem: 448457
  Chemical formula: C20H32O5
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 23 pg/mL
• Specificity: Natural and recombinant General Prostaglandin D2
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: PGD2, Prostaglandin D2, PGD2
• Protein Name: Prostaglandin D2
• Description of Target: Prostaglandin D2 (or PGD2) is a prostaglandin that binds to the receptor PTGDR, as well as CRTH2. It is a major prostaglandin produced by mast cells – recruits Th2 cells, eosinophils, and basophils. In mammalian organs, large amounts of PGD2 are found only in the brain and in mast cells. It is critical to development of allergic diseases such as asthma.