Catalog No: OKCA01821
Size:96 Wells
Price: $857.00
SKU
OKCA01821
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRabbit
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
ELISA Kit PrincipleAviva Systems Biology PROS1 ELISA Kit (Rabbit) (OKCA01821) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PROS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PROS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PROS1 captured in the well.
ELISA Kit Range4.7 - 300 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)9894-102
EDTA Plasma (n=4)8682-91
ELISA Kit Component
ComponentAmount
PROS1 Microplate96 Wells (12 x 8 Well strips)
PROS1 Lyophilized Standard2 x 300 ng
100X Biotinylated PROS1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity1.17 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolPROS1
Gene Full NameVitamin K-dependent protein S
Alias SymbolsPROS, PS21, PS22, PS23, PS24, PS25, PSA, protein S, alpha|protein Sa|vitamin K-dependent plasma protein S
Protein NameVitamin K-dependent protein S
Description of TargetAnticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
Uniprot IDP98118
Protein Accession #XP_002716802.1
Nucleotide Accession #XM_002716756.3
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