Propidium Iodide : PI (OOTA00665)

Catalog No: OOTA00665

Propidium Iodide (PI) is a membrane-impermeant DNA binding dye that cannot penetrate viable cells. PI rapidly enters cells with compromised membranes and intercalates between base pairs allowing exclusion of non-viable cells from analysis of flow cytometry data. PI has a broad emission spectrum from 535-617 nm and can be detected in either FL2 or FL3 detectors. When used with Annexin V FITC, it is recommended to analyze PI in FL2.

Product Info

• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
• Conjugation: PI
• Application: FC
  Excitation Laser: Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
  Emmision: 535-617 nm
• Reconstitution and Storage: Store at 2-8°C and protect from light for 12 months. Do not Freeze.
• Application Info: Propidium Iodide is useful as a viability probe for exclusion of nonviable cells based on light scatter and fluorescence properties. It is recommended to use 10 uL (0.5ug) of Propidium Iodide solution per sample.
• Warning: Propidium Iodide is a potential carcinogen. Protect skin and eyes by wearing suitable protective clothing, gloves and eye/face protection.

Target Info

• Alias Symbols: PI