Catalog No: OOPA00031 (Formerly GWB-BSP062)
Size:2ug
Price: $103.00
SKU
OOPA00031
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for OOPA00031 |
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Product Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
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Host | Escherichia Coli. |
Additional Information | Solubility: It is recommended to Reconstitution: Reconstitute the lyophilized proNT3 in sterile 18M?-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
:: | Product Introduction: proNT-3 (the precursor form of neurotrophin-3) functions through p75NTR and sortilin and induces neuronal apoptosis. In contrast, mature NT-3 interacts with Trk receptors and selectively promotes the survival, growth and differentiation of neurons. Since axon retrograde transport is crucial for neurotrophin signaling, proNT-3 also has an important role in the mechanism of retrograde signaling. Product Description: proNT3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27.4kDa. The proNT3 is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Lyophilized proNT3 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution proNT3 should be stored at 4C between 2-7 days and for future use below -18C. Please prevent freeze-thaw cycles. |
Formulation | proNT3 was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 8.0 and 300mM NaCl. |
Purification | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Purity | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Peptide Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Asn-Met-Asp-Gln. |
Gene Symbol | proNT3 |
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Alias Symbols | Precursor Form Neurotrophin-3, proNT-3. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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