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Datasheets/Manuals | Printable datasheet for PROM1 Antibody (OABB01607) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Flow cytometry|Immunohistochemistry|Western blot |
Additional Information | Notes: WB: The detection limit for PROM1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | E.coli-derived human PROM1 recombinant protein (Position: P531-H865). Human PROM1 shares 61% amino acid (aa) sequence identity with mouse PROM1. |
Purification | Affinity Purified |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Intended Use | For research use only. Not for diagnostic and clinical use. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat Flow Cytometry: 1-3 ug/1x10^6 cells: Human |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Prominin-1(PROM1) detection. Tested with WB, IHC-P, FCM in Human. |
Gene Symbol | PROM1 |
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Gene Full Name | prominin 1 |
Alias Symbols | AC133;antigen AC133;CD133;CORD12;hematopoietic stem cell antigen;hProminin;MCDR2;MSTP061;prominin-1;prominin-like protein 1;PROML1;RP41;STGD4. |
NCBI Gene Id | 8842 |
Protein Name | Prominin-1 |
Description of Target | May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439). |
Uniprot ID | O43490 |
Molecular Weight | 97202 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PROM1 Antibody (OABB01607)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "PROM1 Antibody (OABB01607)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "PROM1 Antibody (OABB01607)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PROM1 Antibody (OABB01607)"?
This target may also be called "AC133;antigen AC133;CD133;CORD12;hematopoietic stem cell antigen;hProminin;MCDR2;MSTP061;prominin-1;prominin-like protein 1;PROML1;RP41;STGD4." in publications.
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What is the shipping cost for "PROM1 Antibody (OABB01607)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PROM1 Antibody (OABB01607)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PROM1 Antibody (OABB01607)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "97202 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PROM1 Antibody (OABB01607)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PROM1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PROM1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PROM1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PROM1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PROM1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PROM1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.