PROM1 Antibody - C-terminal region (OABB01203)

Catalog No: OABB01203

Size:100ug

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Anti-PROM1 antibody, OABB01203, Western blotting
Lane 1: PANC Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: COLO320 Cell Lysate
Lane 5: A549 Cell Lysate

Anti-PROM1 antibody, OABB01203, IHC(P)
IHC(P): Human Tonsil Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Product Format: Lyophilized
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: WB, IHC-P
• Additional Information: Notes: WB: The detection limit for PROM1 is approximately 0.5ng/lane under reducing conditions.
  Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: PROM1(Prominin 1), also called CD133, is a glycoprotein. Maw et al.(2000) noted that D4S1601 and D4S3048, which are located on human chromosome 4p15.3-p15.2, map within intronic sequences of the PROML1 gene. Torrente et al.(2004) reported that a subpopulation of circulating cells expressing AC133, a well-characterized marker of hematopoietic stem cells, also expresses early myogenic markers. Singh et al.(2003) prospectively isolated a CD133-positive cell subpopulation from human brain tumors that exhibited stem cell properties in vitro. However, the true measures of cancer stem cells are their capacity for self renewal and exact recapitulation of the original tumor.
• Reconstitution and Storage: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.. At -20C for one year from date of receipt. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for six months. Avoid repeated freezing and thawing.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PROM1(836-850aa MKNMENGNNGYHKDH), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.
• Purification: Immunogen affinity purified.
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Prominin-1(PROM1) detection. Tested with WB, IHC-P in Human;Rat.

Target Info

• Gene Symbol: PROM1
• Gene Full Name: prominin 1
• Alias Symbols: AC133, Antigen AC133, CD133, CORD12, hematopoietic stem cell antigen, hProminin, MCDR2, MSTP061, PROM1, PROM 1, PROM-1, Prominin-1, Prominin 1, Prominin1, Prominin-like protein 1, PROML1, RP41, STGD4
• Protein Name: Prominin-1
• Description of Target: PROM1(Prominin 1), also called CD133, is a glycoprotein. Maw et al.(2000) noted that D4S1601 and D4S3048, which are located on human chromosome 4p15.3-p15.2, map within intronic sequences of the PROML1 gene. Torrente et al.(2004) reported that a subpopulation of circulating cells expressing AC133, a well-characterized marker of hematopoietic stem cells, also expresses early myogenic markers. Singh et al.(2003) prospectively isolated a CD133-positive cell subpopulation from human brain tumors that exhibited stem cell properties in vitro. However, the true measures of cancer stem cells are their capacity for self renewal and exact recapitulation of the original tumor.
• Uniprot ID: O43490