Prolactin Ovine Protein (OPPA00505)

Catalog No: OPPA00505 (Formerly GWB-E1B611)

Size:1MG

Product Info

• Predicted Species Reactivity: Sheep
• Product Format: Lyophilized from concentrated solution with 0.02-0.03% NaHCO₃
• Additional Information: Protein Content: Protein quantitation was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.93 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC DNAman computer analysis program of protein sequences.
• Reconstitution and Storage: It is recommended to reconstitute the lyophilized Prolactin in sterile 18M-omega-cm H₂O or 0.4% NaHCO₃ adjusted to pH 8-9, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Prl should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purification: The Prolactin n is purified by proprietary chromatographic techniques.
• Concentration: 1 mg/ml (prior to lyophilization)
• Purity: Greater than 99.0% as determined by a) Gel filtration analysis b) analysis by SDS-PAGE
• Biological Activity: Is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
• Peptide Sequence: The sequence of the first five N - terminal amino acids was determined and was found to be Ala - Thr - Pro - Val - Cys - Pro.
• Source: E. coli

Target Info

• Gene Symbol: Prolactin
• Alias Symbols: Mammotropin, Luteotropic hormone, Luteotropin, PRL, Prolactin Ovine, Prolactin Ovine Recombinant
• NCBI Gene Id: 443317
• Protein Name: Prolactin
• Description of Target: Prolactin is a neuroendocrine hormone synthesized primarily by the pituitary gland but also a variety of other cell types including the placenta, brain and uterus. Its primary function is to promote and maintain lactation but has also been shown to have a role in breast cancer development, regulation of reproductive function and immunoregulation. Prolactin Ovine Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids.
• Uniprot ID: P01240
• Protein Accession #: NP_001009306.1
• Protein Size (# AA): Recombinant
• Molecular Weight: 23 kDa