PROK1 ELISA Kit (Human) (OKCD05651)

Catalog No: OKCD05651

Size:96WELLS

PROK1 ELISA Kit (Human) (OKCD05651) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • PROK1 Microplate: 96 Wells (12 x 8 Well strips)
  • PROK1 Lyophilized Standard: 2
  • 100X PROK1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 6.5pg/mL

Target Info

• Gene Symbol: PROK1
• Gene Full Name: prokineticin 1
• Alias Symbols: black mamba toxin-related protein;EGVEGF;EG-VEGF;endocrine-gland-derived vascular endothelial growth factor;mambakine;PK1;PRK1;prokineticin-1.
• NCBI Gene Id: 84432
• Protein Name: Prokineticin-1
• Description of Target: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.
• Uniprot ID: P58294
• Protein Accession #: NP_115790.1
• Nucleotide Accession #: NM_032414.2