PROINSULIN ELISA Kit (Human) (OKEH04011)

Catalog No: OKEH04011

Size:96T

Human PROINSULIN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Proinsulin ELISA Kit (Human) (OKEH04011) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Proinsulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Proinsulin is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Proinsulin captured in the well.
• ELISA Kit Range: 0.625-40pmol/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.9% (n = 20)
  Mean Inter-assay CV%: < 8.9% (n = 20)
• ELISA Kit Component:
  • Anti-Proinsulin Microplate: 96 Wells (12 x 8 Well Strips)
  • Proinsulin Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Proinsulin Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.31pmol/mL
• Specificity: Natural and recombinant Human Proinsulin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PROINSULIN
• Description of Target: Proinsulin is the prohormone precursor to insulin made in the beta cells of the islets of Langerhans, specialized regions of the pancreas. In humans, proinsulin is encoded by the INS gene. Proinsulin is synthesized in the endoplasmic reticulum, where it is folded and its disulfide bonds are oxidized. It is then transported to the Golgi apparatus where it is packaged into secretory vesicles, and where it is processed by a series of proteases to form mature insulin. Mature insulin has 35 fewer amino acids; 4 are removed altogether, and the remaining 31 form the C-peptide. The C-peptide is abstracted from the center of the proinsulin sequence; the two other ends (the B chain and A chain) remain connected by disulfide bonds.