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Progesterone Receptor Antibody (Phospho-Ser190) (OAPC00222)

100 ul
$309.00
In Stock
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Predicted Species Reactivity:
Human
Product Format:
Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Clonality:
Monoclonal
Clone:
1154
Isotype:
IgG1
Host:
Mouse
Application:
WB, IHC
Additional Information:
Packaging :
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Reconstitution and Storage:
For long term storage -20C is recommended. Stable at -20C for at least 1 year.
Replacement Item:
This antibody may replace item sc-270221 from Santa Cruz Biotechnology.
Immunogen:
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser190 of human progesterone receptor.
Purification:
Prepared by affinity purification using a protein G column.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Printable datasheet for OAPC00222
Specificity:
Specific for the ~90k PR-A isoform and the ~120k PR-B isoform phosphorylated at Ser190. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Application Info:
The antibody has been directly tested for reactivity in Western blots with human tissue.
Western blot of whole cell T47D lysate prepared from cells that had been inubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser190. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).
Target Reference:
Attia GR, Zeitoun K, Edwards D, Johns A, Carr BR, Bulun SE (2000) Progesterone receptor isoform A but not B is expressed in endometriosis. J Clin Endocrinol Metab 85:2897-2902.
Clemm DL, Sherman L, Boonyaratanakornkit V, Schrader WT, Weigel NL, Edwards DP (2000) Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody. Mol Endocrinol 14:52-65.
Edwards DP (2005) Regulation of signal transduction pathways by estrogen and progesterone. Annu Rev Physiol 67:335-376.
Lin VC, Woon CT, Aw SE, Guo C (2003) Distinct molecular pathways mediate progesterone-induced growth inhibition and focal adhesion. Endocrinology 144:5650-5657.
Gene Symbol:
PGR
NCBI Gene Id:
5241
Protein Name:
Progesterone receptor
Description of Target:
There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000). Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.
Swissprot Id:
P06401
Protein Accession #:
NP_000917
Nucleotide Accession #:
NM_000926
Molecular Weight:
~90-120 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PGR.
RNA Seq:
Find tissues and cell lines supported by DNA array analysis to express PGR.

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