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Catalog No: OKEH02538
Size:96T
Price: $425.00
SKU
OKEH02538
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02538
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Progesterone ELISA Kit (OKEH02538) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Progesterone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Progesterone and incubated. The Progesterone found in the sample or standards competes with the biotinylated Progesterone for limited binding sites on the immobilized anti-Progesterone antibody. Excess unbound biotinylated Progesterone and sample or standard Progesterone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Progesterone captured in the well and inversely proportional to the amount of Progesterone which was contained in the sample or standard.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 109%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.5% (n = 20)
Mean Inter-assay CV%: < 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Progesterone Microplate96 Wells (12 x 8 Well strips)
Progesterone Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Progesterone-HRP Conjugate1 x 60 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional Information

CAS#: 57-83-0

Molecular Formula: C21H30O2

Molecular Weight: 314.46 g/mol

::Pubchem: 5994
::Chemical formula: C21H30O2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant General Progesterone
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsP4
Protein NameProgesterone
Description of TargetProgesterone also known as P4 (pregn-4-ene-3,20-dione) is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supports gestation) and embryogenesis of humans and other species. Progesterone belongs to a class of hormones called progestogens, and is the major naturally occurring human progestogen. Progesterone is produced in the ovaries (by the corpus luteum), the adrenal glands (near the kidney), and, during pregnancy, in the placenta. Progesterone is also stored in adipose (fat) tissue.
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