Catalog No: OKEH02538
Size:96T
Price: $425.00
SKU
OKEH02538
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | All Species | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Progesterone ELISA Kit (OKEH02538) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Progesterone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Progesterone and incubated. The Progesterone found in the sample or standards competes with the biotinylated Progesterone for limited binding sites on the immobilized anti-Progesterone antibody. Excess unbound biotinylated Progesterone and sample or standard Progesterone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Progesterone captured in the well and inversely proportional to the amount of Progesterone which was contained in the sample or standard. | ||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 109% | ||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.5% (n = 20) Mean Inter-assay CV%: < 9.4% (n = 20) | ||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||
Additional Information | CAS#: 57-83-0 Molecular Formula: C21H30O2 Molecular Weight: 314.46 g/mol | ||||||||||||||||||
:: | Pubchem: 5994 | ||||||||||||||||||
:: | Chemical formula: C21H30O2 | ||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids | ||||||||||||||||||
Sensitivity | 0.056 ng/mL | ||||||||||||||||||
Specificity | Natural and recombinant General Progesterone | ||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Alias Symbols | P4 |
---|---|
Protein Name | Progesterone |
Description of Target | Progesterone also known as P4 (pregn-4-ene-3,20-dione) is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supports gestation) and embryogenesis of humans and other species. Progesterone belongs to a class of hormones called progestogens, and is the major naturally occurring human progestogen. Progesterone is produced in the ovaries (by the corpus luteum), the adrenal glands (near the kidney), and, during pregnancy, in the placenta. Progesterone is also stored in adipose (fat) tissue. |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review