Progesterone ELISA Kit (Fish) (OKCA00351)

Catalog No: OKCA00351

Size:96 Wells

Product Info

• Predicted Species Reactivity: Fish
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: < 4 Hours
• ELISA Kit Linearity:

  Sample Type 1
  Sample Type	Dilution	Average %	Range %
  Serum (n=4)	1:1	94%	85-106%
  Serum (n=4)	1:2	90%	89-99%
  Serum (n=4)	1:4	95%	85-103%
  Serum (n=4)	1:8	90%	87-103%

• ELISA Kit Principle: Aviva Systems Biology PROG ELISA Kit (Fish) (OKCA00351) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PROG and Horseradish Peroxidase (HRP) conjugated PROG. The competitive inhibition reaction is launched between with HRP labeled PROG and unlabeled PROG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PROG in the sample. The color development is stopped and the intensity of the color is measured.
• ELISA Kit Range: 0.4 ng/mL - 30 ng/mL
• ELISA Kit Recovery:

  Sample 1
  Sample Type	Average Recovery	Range
  Serum (n = 5)	0.95	82-107%

  Sample 2
  Sample Type	Average Recovery	Range
  EDTA plasma (n = 4)	0.96	88-102%

• ELISA Kit Reproducibility:

  	Mean Intra-Assay Precision %CV			Mean Inter-Assay Precision %CV
  Sample	1	2	3	1	2	3
  n	20	20	20	20	20	20
  Mean (ng/mL)	2.452	2.519	2.541	2.463	2.474	2.441
  SD	0.039	0.039	0.036	0.049	0.055	0.064
  CV%	3.581	3.627	3.312	4.521	5.037	5.834

• ELISA Kit Component:
  • Anti-PROG Microplate: 96 Well (12 x 8 Well Strips)
  • PROG Standard: 6 x 500 uL
  • Antibody: 1 x 6 mL
  • HRP-Conjugate: 1 x 6 mL
  • 20X Wash Buffer: 1 x 15 mL
  • Substrate A: 1 x 7 mL
  • Substrate B: 1 x 7 mL
  • Stop Solution: 1 x 7 mL
• Additional Information:

  CAS#: 57-83-0

  Molecular Formula: C21H30O2

  Molecular Weight: 314.46 g/mol

• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates
• Sensitivity: < 0.2 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of fish
  PROG. No significant cross-reactivity or interference between fish PROG and
  analogues was observed.
• Assay Info: Assay Methodology: Quantitative Competitive Immunoassay

Target Info

• Gene Symbol: PROG
• Alias Symbols: P4
• Protein Name: Progesterone
• Description of Target: Progesterone also known as P4 (pregn-4-ene-3,20-dione) is a C-21 steroid hormone involved in the female menstrual cycle, pregnancy (supports gestation) and embryogenesis of humans and other species. Progesterone belongs to a class of hormones called progestogens, and is the major naturally occurring human progestogen. Progesterone is produced in the ovaries (by the corpus luteum), the adrenal glands (near the kidney), and, during pregnancy, in the placenta. Progesterone is also stored in adipose (fat) tissue.