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JURKAT Cell Total Protein Lysate (OOSA09789)

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Description of Target:
Application Info:
Western Blotting:    Neat
Additional Information:
Approx Protein Conc: Total protein concentration 2mg/ml
Label Protein Molar Ratio: Jurkat lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin.) Cell debris was removed by centrifugation. The Jurkat lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
Preservative: None present.
Reconstitution and Storage:
Store at 2-8oC for continuous use. For extended storage, freeze working aliquots at -70oC. Repeated freezing and thawing is not recommended.
Printable datasheet for OOSA09789

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