p70 S6 Kinase Antibody (Phospho-Thr389) (OAAF07416)

Catalog No: OAAF07416 (Formerly GWB-ASB191)

Size:100 ug

Western blot analysis of lysates from Jurkat cells treated with Insulin 0.01U/ml 15', using p70 S6 Kinase (Phospho-Thr389) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Thr389) Antibody. The picture on the right is blocked with the phospho peptide.

Immunofluorescence analysis of HUVEC cells, using p70 S6 Kinase (Phospho-Thr389) Antibody. The picture on the right is blocked with the phospho peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid.PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Modification Sites: Human:T412, Mouse:T412, Rat:T412
  
• Reconstitution and Storage: Store at -20°C for 1 year.
• Immunogen: The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr389.
• Purification: The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho
• Specificity: p70 S6 Kinase (Phospho-Thr389) Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Thr389.
• Application Info: WB: 1/500 - 1/2000
  IHC: 1/100 - 1/300
  IF: 1/200 - 1/1000
  ELISA: 1/10000
• Publications:

  Mitochondrial complex I inhibitors suppress tumor growth through concomitant acidification of the intra- and extracellular environment. iScience. 24, 103497 (2021). 34934919

• Description:
  View product citations for p70 S6 Kinase Antibody (Phospho-Thr389) (OAAF07416) on CiteAb

Target Info

• Gene Symbol: RPS6KB1
• Gene Full Name: ribosomal protein S6 kinase B1
• Alias Symbols: 70 kDa ribosomal protein S6 kinase 1;p70 ribosomal S6 kinase alpha;p70 S6KA;p70(S6K)-alpha;p70-alpha;p70-S6K;PS6K;ribosomal protein S6 kinase beta-1;ribosomal protein S6 kinase I;ribosomal protein S6 kinase, 70kDa, polypeptide 1;S6K;S6K1;S6K-beta-1;serine/threonine kinase 14 alpha;serine/threonine-protein kinase 14A;STK14A.
• NCBI Gene Id: 6198
• Protein Name: Ribosomal protein S6 kinase beta-1
• Description of Target: Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR (PubMed:11500364, PubMed:12801526, PubMed:14673156, PubMed:15071500, PubMed:15341740, PubMed:16286006, PubMed:17052453, PubMed:17053147, PubMed:17936702, PubMed:18952604, PubMed:19085255, PubMed:19720745, PubMed:19935711, PubMed:19995915, PubMed:23429703). Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition (PubMed:28178239).
• Uniprot ID: P23443
• Molecular Weight: 59 kDa