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Catalog No: OKEH01954
Size:96T
Price: $600.00
SKU
OKEH01954
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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PRNP ELISA Kit (Mouse) : 96 Wells (OKEH01954)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRNP ELISA Kit (Mouse) (OKEH01954) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRNP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRNP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRNP captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 6.8% (n = 20)
ELISA Kit Component
ComponentAmount
PRNP Microplate96 Wells (12 x 8 Well strips)
PRNP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated S100A10 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.081 ng/mL
SpecificityNatural and recombinant Mouse Major prion protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPrnp
Gene Full Nameprion protein
Alias SymbolsAA960666;AI325101;CD230;major prion protein;P;Prn-i;Prn-p;PrP;PrP;prP27-30;prP33-35C;PrPC;PrPSc;S;Sinc.
NCBI Gene Id19122
Protein NameP04925
Description of TargetMajor prion protein
Uniprot IDhttps://www.uniprot.org/uniprot/P04925
Protein Accession #NP_001265185.1
Nucleotide Accession #NM_001278256.1
Protein Size (# AA)254
Molecular Weight28 kDa
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