PRNP ELISA Kit (Chicken) (OKEH08764)

Catalog No: OKEH08764

Size:96T

Chicken PRNP ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology PRNP ELISA Kit (Chicken) (OKEH08764) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRNP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRNP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRNP captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.5% (n = 20)
  Mean Inter-assay CV%: <=9.5% (n = 20)
• ELISA Kit Component:
  • PRNP Microplate: 96 Wells (12 x 8 Well strips)
  • PRNP Lyophilized Standard: 2
  • 100X Biotinylated PRNP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Its primary physiological function is unclear. Has cytoprotective activity against internal or environmental stresses. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains.
  Subcellular Location: Cell membrane Lipid-anchor GPI-anchor
  Unigene: Gga.3867
  SMR: P27177
  String: 9031.ENSGALP00000040285
  KEGG: gga:396452
  Pfam: PF00377
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.095ng/mL
• Specificity: Natural and recombinant Chicken Major prion protein homolog
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PRNP
• Gene Full Name: prion protein
• Alias Symbols: 65-21 protein;acetylcholine receptor-inducing activity;ARIA;major prion protein homolog;prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia);prion-like-protein;PR-LP;Prn-p;prp.
• NCBI Gene Id: 396452
• Protein Name: P27177
• Description of Target: Major prion protein homolog
• Uniprot ID: https://www.uniprot.org/uniprot/P27177
• Protein Accession #: NP_990796.2
• Nucleotide Accession #: NM_205465.2