PRNP Antibody (OAGA01453)

Catalog No: OAGA01453

Size:100 ul

PRP antibody detects PRNP protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug NIH-3T3 whole cell lysate/extract
C. 30 ug C2C12 whole cell lysate/extract
12 % SDS-PAGE
PRP antibody (OAGA01453) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A:IMR32
12% SDS PAGE
OAGA01453 diluted at 1:1000

PRP antibody detects PRP protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
PRP antibody (OAGA01453) dilution: 1:500.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PRP. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.36mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: PRNP
• Gene Full Name: prion protein
• Alias Symbols: CJD, GSS, PrP, ASCR, KURU, PRIP, PrPc, CD230, AltPrP, p27-30, PrP27-30, PrP33-35C
• NCBI Gene Id: 5621
• Description of Target: The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. An overlapping open reading frame has been found for this gene that encodes a smaller, structurally unrelated protein, AltPrp. Alternative splicing results in multiple transcript variants.
• Uniprot ID: P04156