PRMT3 Antibody (OAGA05430)

Catalog No: OAGA05430

Size:100 ul

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PRMT3 antibody diluted at 1:1000.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
diluted at 1:5000

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
diluted at 1:1000

PRMT3 antibody immunoprecipitates PRMT3 protein in IP experiments.
IP samples: HeLa whole cell extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of PRMT3 protein by 4 ug PRMT3 antibody
7.5 % SDS-PAGE
The immunoprecipitated PRMT3 protein was detected by PRMT3 antibody diluted at 1:500.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PRMT3. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:500-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PRMT3
• Gene Full Name: protein arginine methyltransferase 3
• Alias Symbols: HRMT1L3
• NCBI Gene Id: 10196
• Protein Name: protein arginine N-methyltransferase 3
• Description of Target: This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants.
• Uniprot ID: O60678
• Protein Accession #: NP_005779.1
• Nucleotide Accession #: NM_005788.3