Catalog No: OKEH03751
Size:96T
Price: $275.00
SKU
OKEH03751
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PRL ELISA Kit (Bovine) (OKEH03751) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRL has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PRL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRL captured in well.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Component
ComponentAmount
Anti-PRL Microplate96 Wells (12 x 8 Well strips)
PRL Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PRL Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.24 ng/mL
SpecificityNatural and recombinant Bovine Prolactin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPRL
Gene Full Nameprolactin
Alias SymbolsGHA1;growth hormone A1;Prol;prolactin.
NCBI Gene Id280901
Protein NameP01239
Description of TargetProlactin
Uniprot IDhttps://www.uniprot.org/uniprot/P01239
Protein Accession #NP_776378.2
Nucleotide Accession #NM_173953.2
Protein Size (# AA)229
Molecular Weight26 kDa
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