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Catalog No: OKCD01027 (Formerly GWB-KBBAI6)
Size:96WELLS
Price: $675.00
SKU
OKCD01027
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Datasheets/ManualsPrintable datasheet for PRKG2 ELISA Kit (Human) (OKCD01027)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
90-101%83-104%81-98%91-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, cGMP Dependent Type II (PRKG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, cGMP Dependent Type II (PRKG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, cGMP Dependent Type II (PRKG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Kinase, cGMP Dependent Type II (PRKG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
95-10299
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, cGMP Dependent Type II (PRKG2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, cGMP Dependent Type II (PRKG2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PRKG2 Microplate96 Wells (12 x 8 Well Strips)
PRKG2 Lyophilized Standard2 x 20 ng
100X Biotinylated PRKG2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.127 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Protein Kinase, cGMP Dependent Type II (PRKG2). No significant cross-reactivity or interference between Protein Kinase, cGMP Dependent Type II (PRKG2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPRKG2
Gene Full Nameprotein kinase cGMP-dependent 2
Alias SymbolscGK2;cGKII;cGMP-dependent protein kinase 2;cGMP-dependent protein kinase II;PKG2;PRKGR2;protein kinase, cGMP-dependent, type II;testicular tissue protein Li 99.
NCBI Gene Id5593
Protein NamecGMP-dependent protein kinase 2
Description of TargetCrucial regulator of intestinal secretion and bone growth (By similarity). Phosphorylates and activates CFTR on the plasma membrane. Plays a key role in intestinal secretion by regulating cGMP-dependent translocation of CFTR in jejunum (By similarity). Acts downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapse and increase synaptic plasticity. Phosphorylates GRIA1/GLUR1 at Ser-863 (By similarity). Acts as regulator of gene expression and activator of the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2 in mechanically stimulated osteoblasts. Under fluid shear stress, mediates ERK activation and subsequent induction of FOS, FOSL1/FRA1, FOSL2/FRA2 and FOSB that play a key role in the osteoblast anabolic response to mechanical stimulation (By similarity).
Uniprot IDQ13237
Protein Accession #NP_001269409
Nucleotide Accession #NM_001282480.1
Protein Size (# AA)762
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