PRKAR1A Antibody - C-terminal region (ARP57832_P050)

Catalog No: ARP57832_P050

WB Suggested Anti-PRKAR1A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human Liver

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PRKAR1A
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: MNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLS
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-PRKAR1A (ARP57832_P050) antibody is Catalog # AAPP34326

Target Info

• Gene Symbol: PRKAR1A
• Gene Full Name: Protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
• Alias Symbols: CAR, CNC, CNC1, PKR1, TSE1, ADOHR, PPNAD1, PRKAR1, ACRDYS1
• NCBI Gene Id: 5573
• Protein Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• Description of Target: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.
• Uniprot ID: P10644
• Protein Accession #: NP_997636
• Nucleotide Accession #: NM_212471
• Protein Size (# AA): 381
• Molecular Weight: 43kDa
• Protein Interactions: UBC; RAF1; MAPT; CEP250; GSK3B; AGO3; AGO2; ARPC3; PFDN5; DYRK1B; SET; DYRK1A; PLEKHF2; AKAP10; SOX2; PRKACB; PRKACA; NPM1; CAPN11; RFC2; PPP1CA; SMAD2; HIVEP1; SETD7; PRPF40A; CDK2; CCNE1; SMURF1; Akap7; MEN1; HSP90AA4P; SLC38A5; PRPF31; SAFB; UBD; PATZ1