Catalog No: OKEH04743
Size:96T
Price: $525.00
SKU
OKEH04743
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Prkacb ELISA Kit (Mouse) (OKEH04743) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prkacb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prkacb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prkacb captured in well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Prkacb Microplate96 Wells (12 x 8 Well Strips)
Prkacb Standard2
Sample Diluent1 x 20 mL
Prkacb Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Mouse cAMP-dependent protein kinase catalytic subunit beta
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPrkacb
Gene Full Nameprotein kinase, cAMP dependent, catalytic, beta
Alias SymbolscAMP-dependent protein kinase C beta;cAMP-dependent protein kinase catalytic subunit beta;Cbeta2 PKA;CbPKA;Pka;PKA C-beta;Pkacb.
NCBI Gene Id18749
Protein NameP68181
Description of TargetcAMP-dependent protein kinase catalytic subunit beta
Uniprot IDhttps://www.uniprot.org/uniprot/P68181
Protein Accession #NP_001157670.1
Nucleotide Accession #NM_001164198.1
Protein Size (# AA)351
Molecular Weight41 kDa
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