PRKACA Antibody (Phospho-Thr197) (OAAF00107)

Catalog No: OAAF00107 (Formerly GWB-ASB203)

Size:100 ug

Western blot analysis of extracts from mouse brain, using PKA CAT (Phospho-Thr197) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using PKA CAT (Phospho-Thr197) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human PKA CAT around the phosphorylation site of Thr197.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: PKA α/β CAT (Phospho-Thr197) antibody detects endogenous levels of PKA α/β CAT only when phosphorylated at threonine 197.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000
• Reference: Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259.
  W Li, Learn. Mem., Jan 1996; 2: 320 - 333.
  Sharmin Schauble, J. Biol. Chem., May 2007; 282: 14952 - 14959.
  Yong G. Wang, J. Gen. Physiol., Jan 1998; 111: 113.

Target Info

• Gene Symbol: PRKACA|PRKACB|PRKACG
• Gene Full Name: protein kinase cAMP-activated catalytic subunit alpha|protein kinase cAMP-activated catalytic subunit beta|protein kinase cAMP-activated catalytic subunit gamma
• Alias Symbols: BDPLT19;CAFD1;CAFD2;cAMP-dependent protein kinase catalytic subunit alpha;cAMP-dependent protein kinase catalytic subunit beta;cAMP-dependent protein kinase catalytic subunit gamma;KAPG;PKA C-alpha;PKA C-beta;PKA C-gamma;PKACA;PKACB;PKACg;PPNAD4;protein kinase A catalytic subunit;protein kinase A catalytic subunit beta;protein kinase, cAMP-dependent, alpha catalytic subunit;protein kinase, cAMP-dependent, beta catalytic subunit;protein kinase, cAMP-dependent, catalytic, alpha;protein kinase, cAMP-dependent, catalytic, beta;protein kinase, cAMP-dependent, catalytic, gamma;protein kinase, cAMP-dependent, gamma catalytic subunit;serine(threonine) protein kinase.
• NCBI Gene Id: 5566|5567|5568
• Protein Name: cAMP-dependent protein kinase catalytic subunit alpha|cAMP-dependent protein kinase catalytic subunit beta|cAMP-dependent protein kinase catalytic subunit gamma
• Description of Target: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PubMed:12420224, PubMed:21423175). Phosphorylates GPKOW which regulates its ability to bind RNA (PubMed:21880142).|Phosphorylates a large number of substrates in the cytoplasm and the nucleus.|Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (PubMed:21085490).
• Uniprot ID: P17612|P22612|P22694
• Protein Accession #: NP_001229786.1
• Molecular Weight: 40 kDa