PRKACA Antibody (OAGA00583)

Catalog No: OAGA00583

Size:100 ul

Sample(30 ?g of whole cell lysate)
A:HeLa S3
12% SDS PAGE
OAGA00583 diluted at 1:500

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: C2C12
10% SDS PAGE
OAGA00583 diluted at 1:1000

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PKA alpha(OAGA00583) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2000.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
  Positive Controls: HeLaS3, NIH-3T3, C2C12
  Cellular Localization: Cytoplasm (By similarity), Nucleus (By similarity)
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 351 of PKA C alpha (Uniprot ID#P17612)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: PRKACA
• Gene Full Name: cAMP-dependent protein kinase catalytic subunit alpha
• Alias Symbols: CAFD1, PKACA, PPNAD4
• NCBI Gene Id: 5566
• Protein Name: cAMP-dependent protein kinase catalytic subunit alpha
• Uniprot ID: P17612
• Protein Accession #: NP_002721.1
• Molecular Weight: 41 kDa